Extraction of Trimyristin

Trimyristin is an ester with the chemical formula C45H86O6 that can be uninvolved from the common modify nutmeg. The seed of the evergreen trees in the genus Myristica Fragrans is what nutmeg is obtained from. Trimyristin is the triglyceride of myristic acid also known as saturated fat (3). The variety of trimyristin is solid and is only found soluble in dichloromethane, chloroform, ether, benzene, and ethanol (1). only when twenty to twenty-five percent of trimyristin makes up the mass of nutmeg and it is make from the glycerol and saturated carboxylic acid (2).Like most extractions, isolating trimyristin is colonial considering a variety of products are extracted in the resolving. * Method * Extraction of Trimyristin commencement ceremony the anchor bath was set to about 40o C. In the mean meter 1. 00g of flat coat nutmeg was weighed out and transferred to a 25mL polish bottom flask. Then 10 mL of methylene chloride and 2 boiling chips were added to the round bottom flas k. The round bottom flask was and then connected to an air condenser and get down onto the sand bath.The solution was allowed to boil gently for 30 minutes until it was take away and set aside to feeble. After it cooled the nutmeg residue was filtered from the methylene chloride employ a Hirsch funnel containing a methylene chloride moistened filter paper. A token(prenominal) amount of methylene chloride was then used to wash any lavishness residue out of the round bottom flask and into the filter. The filtrate was then transferred into a clean, dry, and tarred Erlenmeyer flask.The Erlenmeyer flask was then placed on a calefacient plate set between 50oC to 100oC inside the fume hood until the solvent evaporated into a yellowish oily solid. The solid that remained was then weighed. * Recrystallization of Trimyristin Acetone was added in 1mL portions to the Erlenmeyer flask that contained the solid. During the same time, the flask was warmed on a sand bath in order to dissolve the solid. Then it was allowed to slowly cool to room temperature and placed into an ice bath for 10 minutes.The crystals were collected by vacuum filtration and were allowed to dry. * Analysis After the crystals were dry the solid was weighed and then the melt down point was determined using a Melt-Temp machine. * Results Lab Components Mass (g) peck (mL) Nutmeg 1. 00g Flask and Testube 83. 47g Flask, Testube, and separate (initial) 90. 54g Filtrate (initial) 7. 07g Erlenmeyer Flask 74. 14g Erlenmeyer flask and Filtrate (final) 74. 41g Filtrate (final) 0. 26g Acetone 1. 00mL * These results show all the measurements that were taken from everything used in order to determine the analysis of the trimyristin extracted which is shown below. * Analysis Weight of Trimyristin 0. 1g Melting detail of Trimyristin 53. 2oC 54. 3oC % of Trimyristin extracted 10% * Conclusion During this experiment methods of vacuum filtration and recrystallization were used to extract trimyristin from the spice nutmeg.When trimyristin was successfully extracted there was a low percentage (10%) of the amount extracted further it had a melt point that was almost exact to the actual melting point of trimyristin which is 53oC-54oC. The experiment was performed successfully for the most part, disregarding a a few(prenominal) minor mistakes made in the procedure. These mistakes didnt interfere with the outcome of the experiment but could have improved the performance of it. For instance, it in additionk a long time for the solvent to evaporate because the hot plate was set at too low of a temperature.